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KMID : 0613820090190020284
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Journal of Life Science
2009 Volume.19 No. 2 p.284 ~ p.288
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Recombinant Mannose-binding Lectin Protein and Anti-Mannose-binding Lectin Polyclonal Antibody Production
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Kwon Hyun-Mi
Park Jung-Ae
Choi Byung-Tae
Choi Young-Hyun
Chung Kyung-Tae
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Abstract
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The innate immune system is important for the first line of host defence against infectious agents, which have penetrated the mechanical barriers. Mannose-binding lectin (MBL or mannan-binding protein, MBP) is a serum protein that is synthesized in the liver as a part of the acute phase response. MBL binds to carbohydrate structures presented by a wide range of pathogenic bacteria, viruses, fungi, and parasites. MBL is synthesized as a monomer that has a carboxy-terminal carbohydrate recognition domain, a neck region and a collagen region. Low MBL level was reported to be the most frequent immuno-deficiency syndrome. Although extensive studies have yielded detailed information on the structure of MBL, functions of the MBL complex are not fully understood yet. We, here, present cloning process of MBL cDNA from the rat liver and production of truncated recombinant MBL protein using a bacterial expression system in order to produce anti-MBL polyclonal antibody. Anti-MBL polyclonal antibody was raised in a New Zealand rabbit and its affinity was tested against recombinant protein using western blot technique. MBL cDNA, recombinant protein and anti-MBL antibody could be used as great arsenals to dissect cellular biochemistry of MBL.
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KEYWORD
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Mannose-binding lectin, MBL, mannose-binding protein, MBP in Korea
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